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ImmunoTools gm-csf cytokine
Gm Csf Cytokine, supplied by ImmunoTools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gm-csf+cytokine/gm+csf+cytokine/pm40660361-106-26-33
Average 90 stars, based on 1 article reviews
gm-csf cytokine - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: C9orf72 Repeat Expansion Induces Metabolic Dysfunction in Human iPSC-Derived Microglia and Modulates Glial–Neuronal Crosstalk
Article Snippet: After 3–4 weeks, floating cells were collected and seeded at 100,000 cells/cm2 on Matrigel-coated plates in Advanced DMEM/F-12 (Life Technologies) supplemented with N2 (Thermo Fisher), GlutaMAX, P/S, β-mercaptoethanol, 100 ng/mL M-CSF, 100 ng/mL IL-34 (PeproTech) and 10 ng/mL GM-CSF (ImmunoTools), with the medium changed twice weekly.

Article Title: Chemoresistant tumor cell secretome potentiates immune suppression in triple negative breast cancer.
Article Snippet: In brief, monocytes were cultured for 6 days in complete RPMI medium supplemented with recombinant human cytokines; 50 ng/mL M-CSF to generate MΦs and 70 ng/mL GM-CSF and 50 ng/mL IL-4 (all from ImmunoTools) to generate DCs.

Article Title: The role of mRNA-galsomes and LNPs in enhancing HIV-specific T cell responses across various lymphoid organs
Article Snippet: BMDCs were generated by culturing bone marrow from CD45.1 + mice with 20 ng/mL GM-CSF (Immunotools, Friesoythe, Germany) for 6 days in RPMI, 5% SUP, 5% fetal clone I serum (FCI) (Thermo Fisher Scientific, Brussels, Belgium) and 50 μM β-mercaptoethanol (from here on referred to as RPMI+).

Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia
Article Snippet: Isolated cells were cultured in complete DC media composed of IMDM and glutamaxTM (Gibco) with 10% FBS, 50 ng/ml GM-CSF (ImmunoTools, Friesoythe, Germany), 1% penicillin/streptomycin, 1% L-glutamine (Sigma-Aldrich) and 2-mercaptoethanol (Gibco).

Article Title: Soluble isoforms of the DC-SIGN receptor can increase the dengue virus infection in immature dendritic cells
Article Snippet: For dendritic cell differentiation, 10 4 cells of THP-1 were plated per well in a 96-well plate, with RPMI medium supplemented with 10 % BFS and cytokines for differentiation, GM-CSF (50 ηg/mL; Immunotools), and IL4 (50 ng/mL; Immunotools).

Article Title: Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma
Article Snippet: After 3 h of incubation at 37 °C the supernatant containing non-adherent cells was replaced with fresh DC-medium supplemented with 1000U/ml GM-CSF and 1000 U/ml IL-4 (ImmunoTools, Friesoythe, Germany).

Article Title: The role of mRNA-galsomes and LNPs in enhancing HIV-specific T cell responses across various lymphoid organs
Article Snippet: BMDCs were generated by culturing bone marrow from CD45.1+ mice with 20 ng/mL GM-CSF (Immunotools, Friesoythe, Germany) for 6 days in RPMI, 5% SUP, 5% fetal clone I serum (FCI) (Thermo Fisher Scientific, Brussels, Belgium) and 50 mM b-mercaptoethanol (from here on referred to as RPMI+).

Article Title: Streptokinase is dispensable in Streptococcus dysgalactiae subspecies equisimilis infections of human dendritic cells
Article Snippet: Monocytes were incubated for 5 d in RPMI1640 (Cytivia) medium supplemented with 10% (v/v) heat-inactivated fetal calf serum (Thermo Fischer), 89 ng/ml GM-CSF (Immunotools), and 22 ng/ml IL-4 (Immunotools) to generate moDCs.

Cell Culture:

Article Title: C9orf72 Repeat Expansion Induces Metabolic Dysfunction in Human iPSC-Derived Microglia and Modulates Glial–Neuronal Crosstalk
Article Snippet: After 3–4 weeks, floating cells were collected and seeded at 100,000 cells/cm2 on Matrigel-coated plates in Advanced DMEM/F-12 (Life Technologies) supplemented with N2 (Thermo Fisher), GlutaMAX, P/S, β-mercaptoethanol, 100 ng/mL M-CSF, 100 ng/mL IL-34 (PeproTech) and 10 ng/mL GM-CSF (ImmunoTools), with the medium changed twice weekly.

Article Title: Chemoresistant tumor cell secretome potentiates immune suppression in triple negative breast cancer.
Article Snippet: In brief, monocytes were cultured for 6 days in complete RPMI medium supplemented with recombinant human cytokines; 50 ng/mL M-CSF to generate MΦs and 70 ng/mL GM-CSF and 50 ng/mL IL-4 (all from ImmunoTools) to generate DCs.

Article Title: The role of mRNA-galsomes and LNPs in enhancing HIV-specific T cell responses across various lymphoid organs
Article Snippet: BMDCs were generated by culturing bone marrow from CD45.1 + mice with 20 ng/mL GM-CSF (Immunotools, Friesoythe, Germany) for 6 days in RPMI, 5% SUP, 5% fetal clone I serum (FCI) (Thermo Fisher Scientific, Brussels, Belgium) and 50 μM β-mercaptoethanol (from here on referred to as RPMI+).

Article Title: Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia
Article Snippet: Isolated cells were cultured in complete DC media composed of IMDM and glutamaxTM (Gibco) with 10% FBS, 50 ng/ml GM-CSF (ImmunoTools, Friesoythe, Germany), 1% penicillin/streptomycin, 1% L-glutamine (Sigma-Aldrich) and 2-mercaptoethanol (Gibco).

Article Title: Soluble isoforms of the DC-SIGN receptor can increase the dengue virus infection in immature dendritic cells
Article Snippet: For dendritic cell differentiation, 10 4 cells of THP-1 were plated per well in a 96-well plate, with RPMI medium supplemented with 10 % BFS and cytokines for differentiation, GM-CSF (50 ηg/mL; Immunotools), and IL4 (50 ng/mL; Immunotools).

Article Title: Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma
Article Snippet: After 3 h of incubation at 37 °C the supernatant containing non-adherent cells was replaced with fresh DC-medium supplemented with 1000U/ml GM-CSF and 1000 U/ml IL-4 (ImmunoTools, Friesoythe, Germany).

Article Title: The role of mRNA-galsomes and LNPs in enhancing HIV-specific T cell responses across various lymphoid organs
Article Snippet: BMDCs were generated by culturing bone marrow from CD45.1+ mice with 20 ng/mL GM-CSF (Immunotools, Friesoythe, Germany) for 6 days in RPMI, 5% SUP, 5% fetal clone I serum (FCI) (Thermo Fisher Scientific, Brussels, Belgium) and 50 mM b-mercaptoethanol (from here on referred to as RPMI+).

Article Title: Streptokinase is dispensable in Streptococcus dysgalactiae subspecies equisimilis infections of human dendritic cells
Article Snippet: Monocytes were incubated for 5 d in RPMI1640 (Cytivia) medium supplemented with 10% (v/v) heat-inactivated fetal calf serum (Thermo Fischer), 89 ng/ml GM-CSF (Immunotools), and 22 ng/ml IL-4 (Immunotools) to generate moDCs.



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